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WIN55, 212-2 increased the level of BDNF mediated PKAC-â and C-FOS in cerebral cortex after ICH in rats

Li Zhu, Min Li, Mingqing Peng, Zhi Dong, Zhonglin Wang, Jiahui Ding, Chun Tian

To observe whether cannabinoid receptor (CB1R) agonist improve neurological function accompanied by an increase in Brain-Derived Neurotrophic Factor (BDNF), mediated by C-FOS and a decrease in PKA C-â in cerebrum cortex after intracerebral hemorrhage (ICH) in rats. Intracerebral hemorrhagemodel of ratwasmade byVII Collagenase,which was killed for brain tissue as specimens with 24 hours. The mRNA levels of PKAC-â, C-FOS and BDNF expression were detected by semiquantitative RT-PCR. The expression of PKAC-â, C-FOS and BDNF proteins were determined byWestern blotting and immunohistochemistry. Intraperitoneal (i.p.) injection ofWIN55-212-2 could obviously improve some nervous deficit symptoms and increased the mRNA and protein levels of BDNF. Increased BDNF synthesis was accompanied by increased activation of C-FOS and downregulation of PKA in the ipsilateral cerebral cortex prepared from WIN55-212-2 rats. PKAC-â, C-FOS, and BDNF proteins are expressed on membrane of neuron, nucleus of neuron or the cytoplasm of glial cells respectively. The expression of BDNF is induced not only by activation of C-FOS, but also by inhibiton of PKA through stimulation ofMAP kinase inWIN55, 212-2 rats