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Validated RP-HPLC method for estimation of diacerein and aceclofenac in tablet dosage form

Nikunjkumar Patel, Prachi Kabra, Ritu Vivek Kimbahune, Pratik Patel, L.V.G.Nargund

In the present study, a reverse phase high performance liquid chromatographic method was developed and validated for the determination of Diacerein (DIA) andAceclofenac (ACE) in tablet dosage formwith the use of Paracetamol (PCM) as an internal standard (IS). Chromatographic separation was carried out on a RP-18 column using amobile phase consisting of acetonitrile : water (60:40, v/v) 0f pH 3.0,adjusted with o-phosphoric acid (1%). The flow rate was maintained at 1.0ml/min and UV detection was carried at 268nm. The calibration curve was found linear over the range 10-150µg/ml for DIA and 20-300µg/ml. Relative Standard deviation (RSD) for precision was found to be less than 3%. The results of accuracy study were observed in the range of 99.50%to100.18%withRSD less than 1%. Limit of detection (LOD)was found to be 0.9839µg/ml for DIA and 1.9479µg/ml for ACE. Limit of quantification (LOQ)was found to be 2.9816µg/ml for DIA and 5.9030µg/ ml forACE. The developed analyticalmethod was found to be simple, rapid, and easy to apply, making it suitable for routine analysis ofACE and DIAin tablet dosage form.