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Validated HPTLC Method for Simultaneous Estimation of Domperidone in Combination with Rabeprazole Sodium in Solid Dosage Form

Vidya Gawande and Manisha Puranik

The present work describes high performance thin layer chromatographic method for simultaneous estimation of domperidone in combination with rabeprazole sodium in capsule formulation. Chromatography was performed on (10 ×10 cm) silica gel F254 TLC plates using mobile phase toluene : methanol (9 : 1 v/v) with 15 min time of saturation with filter paper. Chromatographic conditions were found to effectively separate domperidone (Rf = 0.12 ± 0.0041) and rabeprazole sodium (Rf = 0.17 ± 0.0052). Standard calibration curve was found to be linear in the range 0.06-0.3 Ag/spot for domperidone and 0.04-0.2 Ag/spot for rabeprazole sodium,, respectively. The proposed method was found to be accurate, precise, reproducible, economic, reliable and specific and can be used for simultaneous analysis of these drugs in capsule formulation.