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Time regulation researches on degrading reactive dyes by immobilized white rot fungi under nonsterile RESEcondition

Yonggang Zeng, Dawen Gao, Hong Liang

The effects and mechanisms on the decolorization of reactive dyes K-2BP at the different dye-added time were investigated in this study under non-sterile conditions. The results showed that based on the optimal bacteriostasis incubation strategy for immobilization, the decolorization efficiency achieved 94.69% and 93.65% with 2 and 3 days cultivation after adding dye. The cultivation and degradation cycle were shortened from the original 10 days to 4-5 days. However the maximum decolorization efficiency was only 57.56% in the 5th day dye-added system. The lower decolorization efficiency was due to the peak of enzyme production was missed by adding dye after 5 days cultivation, instead of the disturbance of other bacterias. Therefore, the optimal bacteriostasis incubation strategy for immobilization under non-sterile conditions could successfully restrain the growth of other bacterias. Furthermore accompanied with the appropriate dye-added time, the degradation cycle on the reactive dyes can be shortened.