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The Qualitative and Quantitative Assay of Siderophore Production by some Microorganisms and Effect of Different Media on its Production

Swapan Kumar Ghosh, Sujoy Pal and Niloy Chakraborty

The production of siderophore by biocontrol agents (BGA) and plant growth promoting microbes (PGPM) is one of the important mechanisms for plant growth promotion and disease suppression. Microorganisms compete for iron by releasing siderophores. In this experiment, three fungi (Trichoderma viride-1 and T. harzianum-1 and Candida famata-1) and three bacteria (Bacillus subtilis-1, B. megatericus 1, Pseudomonas aeroginosa1) are taken for their evaluation as siderophore producer by both qualitative and quantitative assay. All fungi and bacteria gave positive response to qualitative assay. In quantitative assay, among the fungi, C. famata gave maximum (60.00%) siderophore while among the bacteria and fungi, P. aeroginosa yielded highest (80.50) percentage of siderophore. Moreover, effect of different media (MEB, NB, SMB, BRB and CCAB) on the siderophore production of P. aerogenosa was recorded where MEB supported maximum percentage of siderophore production (80.50%) but NB did not support. In modern science, production of pure siderophore in commercial way is very necessary as application of siderophore is in increasing trends in agriculture, medical science etc. Therefore, this work may be helpful for mass production of siderophore from microbes.