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The Increasement of Rice Straw Hydrolysis using Blend Crude Cellulose Enzyme from Trichoderma reesei and Aspergillus niger

Bambang Dwi Argo, Agustin Krisna Wardani, Elok Zubaidah and Sri Winarsih

The crude enzyme from Trichoderma reesei and Aspergillus niger have different activities. The use of two crude enzyme separately only able to produce a low levels of glucose, but after the two crude enzyme being mixed, it is able to improve the yield of glucose on cellulose hydrolysis process by using rice straw as substrate which has undergone pretreatment by alkali microwave. The combination of crude enzyme from Trichoderma reesei and Aspergillus niger (2:1) has the FP-ase activity of 1,002 IU/ml, CMC-ase activity of 2.23 IU/ml and Alpha-glucosidase activity of 0.168 IU/ml. The activity of the crude enzyme cellulose combination are able to generate sugar at 12.89 mg/ml in the process of hydrolysis for 72 hours.