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Study of the combination reaction between drugs and bovine serum albumin with acriflavine as a fluorescence probe

Ying Guo, Baosheng Liu, Zhiyun Li, Lihui Zhang, Yunkai Lv

Using Acriflavine as fluorescent probe to study the seven kinds of drugs to combination of the bovine serumalbumin. These drugs are Streptomycin sulfate, Kanamycin sulfate, Gentamicin, Amikacin, Neomycin, Thiamphenicol, and Florfenicol.And after reaction of bovine serumalbumin and these drugs, the fluorescence intensity of bovine serum albumin has no obvious change. Research has shown that after adding seven kinds of drugs to the bovine serum albumin and Acriflavine system, respectively, the fluorescence intensity of bovine serum albumin has recoveried. The degree of recovery are GM> TN> NM> FN> KM> SM> AM. It can be concluded that the seven kinds of drugs specifically binds to bovine serum albumin, and it determine the binding site on sub-domain IIA (site I) of the seven kinds of drugs and bovine serum albumin.