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Spectrophotometric determination for the analysis of cefuroxime axetil in pharmaceutical dosage forms

K.Pavankumar, T.Praba, M.Jagadeeswaran, A.Caroline Grace, T.Sivakumar

Three simple and accurate spectrophotometric methods have been developed for the estimation of CefuroximeAxetil in bulk drug and its solid dosage forms. Method A was described about UV spectrophotometric measurement atmaximumabsorption of 277 nmwhileMethod B related to formation of green colored chromogen by utilizing the oxidative coupling reaction between MBTH and Cefuroxime Axetil in presence of ferric chloride. It was measured at 624 nm against reagent blank. Method C was based on the formation of pink colored chromogen with PDAC and it was measured at 537 nm. The conditions of reaction pathway affecting the varied parameterswere studied thoroughly and optimized. Under optimized conditions, the beerÂÂ’s law obeyed at the concentration of 5-25 µg/ml with correlation coefficient of 0.999 (n=5), 1-5 µg/ml with correlation coefficient of 0.9992 (n=5) and 2-10 µg/ml with correlation coefficient of 0.998 (n=5) for Method A, B and C respectively. The results of validated parameters like linearity, precision, accuracy, robustness and ruggedness compiled with those of official methods. There is no interference found from tablet excipients at the selected wavelength (Method B & C). The developed analysis could be considered successfully for the determination of CefuroximeAxetil in pharmaceutical formulation.