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Spectrofluorimetric determination of flupentixol dihydrochloride and quetiapine in pharmaceutical preparations and spiked human plasma via oxidation with cerium (IV)

N.El-Enany, F.Belal, A.El-Brashy, N.El-Bahy

A simple and sensitive kinetic spectrofluorometric method was developed for the determination of flupentixol (FPX) andQuetiapine (QTP). Themethod was based upon oxidation of the studied drugs with cerium(IV) ammonium sulphate in acidic medium. The fluorescence of the produced Ce (III) was measured at 362 nmafter excitation at 255 nm. The fluorescence –concentration plots were rectilinear over the concentration ranges of 0.04 to 0.2 µg/ml-1 and 0.025 to 0.6 µg/ml-1 withLODof 1.7 x 10-3 and 7.7 x 10-4 µg/ml-1 and LOQ of 5.2 x 10-3 and 2.3 x 10-3 µg/ml-1 for FPX and QTP respectively. The method was successfully applied to the analysis of commercial tablets and ampoules. The results obtained were in good agreement with those obtained with reference methods. The mean%recoveries of FPXin tablets and ampoules (n=5) were 100.22 ± 0.75 and 99.70 ± 0.58 for tablets and ampoules respectively. The mean % recoveries of QTP in tablets (n=5) were 101.09 ± 0.74. The high sensitivity attained by the proposed method allowed the determination of QTP in spiked human plasma. The mean % recoveries ofQTP in spiked human plasma (n=3) were 97.43 ± 0.81.