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Simultaneous Estimation of Atenolol and Hydrochlorothiazide in Combined Dosage Form by UV-Spectrophotometric Methods

Manzoor Ahmed, Nadeem Jamadar and A. Satishkumar Shetty

Two simple, precise and accurate derivative spectrophotometric methods have been developed and validated for the simultaneous estimation of Atenolol (ATN) and Hydrochlorothiazide (HCTZ) in bulk and pharmaceutical formulation. Method A is Area Under Curve method, which involved the measurement of area between 221 to 231 nm and 265 to 275 nm for the estimation of Atenolol and Hydrochlorothiazide respectively. Method B applied Second order derivative spectrophotometry, which involved measuring the absorbance values at 226 nm and 270 nm of second derivative spectrum, without mutual interference with a linearity range of 2-10 μg/mL and 1-5 μg/mL for the estimation of Atenolol and Hydrochlorothiazide, respectively for both the methods. Results of analysis were statistically reported and were found to be satisfactory