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Simultaneous estimation of Aspirin and Ticlopidine hydrochloride in combination by derivative spectroscopy.

Syed Fazlur Rahman, Ritu Vivek Kimbahune, G.Mubeen, Shaik Mohammed Maaz, Prachi Kabra

Two methods for simultaneous estimation of Aspirin and Ticlopidine hydrochloride in combination have been developed using 0.1N hydrochloric acid as a solvent. The sample solution was scanned in the range 190-400nm at fast speed. The resulted spectrum was converted into first and second order derivative (using Ä ë=16, scaling factor 100). In second order derivative spectroscopy Aspirin was measured at 303nm while Ticlopidine hydrochloride was determined at 288nm. In second method, amplitudes of first order derivative were measured at 279.2 and 288.2nm and the concentration of Ticlopidine andAspirin were estimated by using simultaneous equation method. The linearity ranges for Aspirin and Ticlopidine hydrochloride were found to be 20-200µg/ml and 100- 700µg/ml respectively at 279.2nm, 288.2nm for first order and 288nm, 303nm for second order derivative method. The accuracy of the methods was assessed by recovery studies was found to be 95.95% and 101.49% for second order derivative method and112.22%and 99.14%for simultaneous equation method for Aspirin and Ticlopidine hydrochloride respectively. These methods have been found to be simple, accurate and rapid; does not require preliminary separation and can therefore be used for routine analysis of both drugs in quality control laboratories.b