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Significance of vitamins, substrate concentration and agitation on biotransformation of anti-inflamatory meloxicam by Cunninghamella blakesleeana

G.Shyam Prasad, S.Girisham, S.M.Reddy

Influence of vitamins, substrate concentration and shaking speed on biotransformation ofmeloxicamwas studied by employingCunninghamella blakesleeana NCIM687 with an aimto achieve maximumtransformation ofmeloxicamand in search of newmetabolites. Based on HPLC, LC-MS/ MS data and previous reports the metabolites were predicted as 5- hydroxymethylmeloxicam, 5-carboxymeloxicamand a novelmetabolite. The quantification of metabolites was performed using HPLC peak areas. The results obtained indicate that folic acid as vitamin source, 0.01%concentration of meloxicamand 120rpmwere found to be optimumfor maximumtransformation ofmeloxicam. The study suggests the significance of these factors in biotransformation ofmeloxicamusingmicrobial cultures.