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RP-HPLC Determination Of Paracetamol, Guaifenesin And Dextromethorphan In Liquid Dosage Form

S.N.Meyyanathan, U.Sivakumar, G.Padmanaban, K.Gopal, S.Muralidharan, S.Rajan

A simple, economical, rapid, reliable and precise reverse phase high performance liquid chromatographic method has been developed for the simultaneous estimation of paracetamol, guaifenesin and dextromethorphan in syrup dosage form. The separation was achieved on a Phenomenex C 18 ( 250 × 4.6 mm ) 5μ column in isocratic mode with water: methanol: glacial acetic acid (60:38.5:1.5 v/v) as mobile phase at a flow rate of 1.5ml/min.The detection was carried out at 276nm.The retention time of paracetamol, guaifenesin and dextromethorphan were found to be 3.20, 5.80 and 9.45 min, respectively. This developed method was validated in terms of accuracy, precision, linearity, limit of detection, limit of quantitation and solution stability. The proposed method can be used for the estimation of these drugs in combined dosage forms in routine analysis.