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Role of isozymes in systematic study of Tephrosia purpurea, Tephrosia villosa and Tephrosia spinosa

Johnson M, D.Patric Raja

Selected isozymes were investigated in the plants Tephrosia purpurea (L.)Pers, Tephrosia villosa (L.)Pers. and Tephrosia spinosa (L.f.)Pers. The standard technique of vertical poly acrylamide gel electrophoresis was employed. The gels were stained for different enzymes systems viz. esterase, peroxidase, acid phosphatase, alkaline phosphatase and polyphenol oxidase. The isozymes were fixed by using the standard procedure, the number, position and polymorphisms were recorded. The present study produced the biochemical markers for these three important weedy medicinal plants. The similarity index and variations between these three species are reported with reference to enzymes esterase, peroxidase, acid phosphatase, alkaline phosphatase and polyphenol oxidase.