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Quantitative HPLC determination of itaconic acid residuals in polymer hydrogels

Palapparambil Sunny Gils, Debajyoti Ray, Prafulla Kumar Sahoo

A simple reversed-phase high-performance liquid chromatographic (RPHPLC) method has been developed and optimized for simultaneous determination of itaconic acid in polymer hydrogels. Separation of itaconic acid was successfully achieved on a C18 column utilizing aqueous solution of ortho-phosphoric acid (0.05 %). The detection was achieved using PDA detector at 210nm. The extraction recovery of itaconic acid was >89%and the calibration curvewas linear (r2 = 0.9990±0.06) over itaconic acid concentrations ranging from0.1 to 1.0g/ml (n = 5).Adequate assay for intra- and inter-day precision and accuracy was observed during validation process. An extraction procedure to remove residual itaconic acid was also established. The developedmethod and the extraction procedure were applied to itaconic acid based hydrogels, tested with or without post polymerization treatment and proved to be accurate and precise for determination of residual monomer content of the material evaluated.