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Quantitative estimation of cilostazole in rat plasma by HPLC-ms/ms and its application to pharmacokinetic study

Thejo moorthy.Karavadi, Bala Sekhara Reddy.Challa

Authors developed a simple, highly sensitive, selective, rapid, rugged and reproducible liquid chromatography–tandem mass spectrometry method for the quantification of Cilostazole (CZ) in rat plasma using Cilostazole d4(CZIS) as an internal standard (IS). Chromatographic separation was performed on Princeton Spher-100 C18, 50 × 4.6 mm, 5 µm column. Mobile phase composed of 0.1%formic acid: acetonitrile (40:60 v/v), with 0.6 mL/ min flow-rate. Drug and IS were extracted by Liquid- liquid extraction. CZ and CZIS were detected with proton adducts at m/z 370.2ï‚®124.9 and 374.3ï‚®125.1 in multiple reaction monitoring (MRM) positive mode respectively. The method was validated with the correlation coefficients of (r2) e” 0.9950 over a linear concentration range of 0.5-1500.0 ng/mL. This method demonstrated intra and inter-day precision within 1.00 to 3.73 and 1.25 to 4.45%and accuracywithin 97.33 to 100.16 and 92.0 to 99.42%for CZ. This method is successfully applied in the Pharmacokinetic study of male rats.