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Quantification Of Atropine In Rat Plasma By Liquid Chromatography Tandem Mass Spectrometry

Yong Chen, Xinli Wang, Huaixia Chen, Fengmei Han, Peng Du

An analytical method based on liquid chromatography coupled with electrospray ionization (ESI) ion trap tandem mass spectrometry detection has been developed and validated for the determination of atropine in rat plasma using anisodamine as the internal standard in selected reaction mornitoring (SRM) mode. The standard curve was linear in the range of 5 to 5000 ng/mL. The assay was specific, accurate, precise and reproducible. The method was employed in a pharmacokinetic study after a single oral gavage dose of 50 mg/kg atropine to six rats.