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Production and optimization of lipase enzyme by Pseudomonas sps.

G.Narasimha, A.Praveen Kumar, D.Subramanyam

Lipase (Triacylglycerol acylhydrolase, E.C. 3.1.1.3) is one of thewell known industrial useful enzymes as it can catalyze different reactions including hydrolysis of triglycerides, transesterification and chiral synthesis of esters under natural conditions. In the present study, the lipase enzyme producing bacterial strainwas isolated fromsoil contaminatedwith groundnut oil and identified as Pseudomonas sps based on its morphological, physiological and biochemical characteristics. The optimal parameters for lipase enzyme production were carried out by using different carbon and nitrogen sources in the medium maintained at pH-7 through submerged fermentation. Various chemical and physical conditions carried out in the present study, Olive oil, Glucose andAmmonium sulphate were served as best carbon and nitrogen sources for lipase production by Pseudomonas sps grown at 30ï‚°C during 24h incubation period respectively.