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Poly D, L-lactide-co-glycolic acid (PLGA)-encapsulated CpG-oligo nucleotide (ODN) on immune response in Cyprinus carpio against Aeromonas hydrophila

Govintharaj Yogeshwari, Chandrasekar Jagruthi, Ramasamy Harikrishnan

The effect of Poly D, L-lactide-co-glycolic acid (PLGA) encapsulated ODN 1668 nanospheres (NS) on innate and adaptive immune response in common carp, Cyprinus carpio against Aeromonas hydrophila is reported. PLGA-encapsulated ODN biodegradable NS did not have a significant impact on particle size as expected of the particles was clearly positive for non-coated and coated PLGA/ODN NS. The myeloperoxidase (MPO) activity and serum haemolysin titre significantly increased in fish injected with ODN 1668 and PLGA-encapsulated ODN 1668 groups on week 4 whereas the respiratory burst (RB) activity significantly increased when administered with ODN 1668 and PLGA-encapsulated ODN 1668 (PLGA-ODN 1668) from weeks 1 to 4. The haemoagglutinating titre was significantly enhanced in PLGA-encapsulated ODN 1668 group on week 2 while in ODN 1668 and PLGA-encapsulated ODN 1668 groups the increase manifested on week 4. The bacterial agglutination titre significantly increased inODN 1668 and PLGA-encapsulated ODN 1668 groups on weeks 2 and 4. The cumulative mortality in ODN 1668 and PLGAencapsulated ODN 1668 groups were 10% each whereas it was 15% in PLGA group. This study indicates that single administration of PLGAencapsulated ODN 1668 nanospheres elicits better immune response than PLGA or ODN 1668 alone in C. carpio against A. hydrophila.