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Partition coefficient studies of calotropain from its crude latex using ionic liquid based aqueous two phase extraction system

Senthilkumar Rathnasamy, R.Kumaresan

Separation and purification of high value bio-molecules using Ionic liquid (IL) based aqueous two phase (ATP) extraction is an emerging green separation process. IL based ATP system is the best alternative for the conventional organic solvent extraction and polymer basedATP systems. The current study is to compare the partition coefficient of calotrpin from the latex of medicinal plant Calotropis procera (Family-Asclepiadaceae; The molecular mass and iso-electric point of the enzyme are 28.8 kDa and 9.32, respectively) and the same was compared with conventional ATP system. The resulting protein was purified on Gel filtration chromatography column and it is confirmed with SDS page analysis. From the results it was found that IL based ATP system is highly selective and yield higher than the conventional ATP system.