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Optimization of fermentation conditions for human collagen a1(iii) chain production by pichia pastoris GS115 using response surface methodology

Lin B.Li, Dai D.Fan, Xiao X.Ma, Pei Ma, Jian J.Deng

The optimal fermentation conditions for recombinant human collagen a1 (III) chain (rhCOL3A1) production by Pichia pastoris GS115 in a shake-flask culture was studied using statistical experimental design and analysis. The response surface methodology (RSM) was effective in optimizing conditions using the limited number of experiments. The optimal fermentation conditions for maximum rhCOL3A1 yield, was determined on the basis of a three-level three-factor Box-Behnken design (BBD), obtained by RSM. The high correlation between the observed and predicted values indicated the validity of the model. A maximum rhCOL3A1 yield of 508.46 mg/L was obtained at 28.2 oC, initial pH 4.95 and 1.35%/24h of methanol, which was 18.9% higher than production of 427.63 mg/L before optimization.