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Optimization of Alkaline Protease Production Medium Components and Physical Parameters by Bacillus Cereus Strain P5 Through Central Composite Design

Sreedevi B, Chandrasekhar K and Pramoda Kumari J

Optimization of the medium for enhanced alkaline protease production was studied using Bacillus cereus P5. Central composite design was applied to optimize the medium components and study the effects of four medium constituents, such as fructose, beef extract, incubation time and pH. The optimum values for the tested variables were fructose 2.8%, beef extract 1.5%, incubation time 72 h and pH 9.0. The model was very sensitive as the coefficient of determination was 0.94. Experiments were validated to verify the sensitivity of the model and the observed values follow the predicted values obtained by CCD. The enzyme production was enhanced up to 1538 U mL−1 at 72 h of incubation and 1.64-fold increase in enzyme production was observed.