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Myristic acid methyl ester: A potential quorum quencher from Melia dubia against uropathogenic E. coli

S.Adline Princy, R.Vinothkannan, D.Navya Rajesh, D.Priyadarshini, V.Praveen Krishna

SdiA, transcriptional quorum sensing (QS) regulator controls the behavioral changes of uropathogenic E. coli in establishing biofilm and virulence. SdiA selective inhibitions characterized fromthe leaf ethanolic extract of Melia dubia showed > 50% inhibition of UPEC on biofilm in a time dependentmanner without affecting cell growth. The compound C39 (MyristicAcidMethyl Ester, obtained fromGC-MS) was found to have GScore of 9.6 as it interactswithTYR67, SER138, SER47 andTRP 71 residues via hydrogen bonds. The in vitro validation of C39 mode of action showed SdiA selective inhibition with mutants and wild types. The data was invariably higher than the existing SdiAselective inhibitor, furanone and this was confirmed by the fluorescence microscopy studies.