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Method Development And Validation For Terbutaline Sulfate, Guaifenesin And Bromhexine Hydrochloride In A Syrup Formulation By Ion Pair RP-HPLC

M.Vijeyaanandhi, R.Vasuki, R.Maheswari, P.Shanmugasundaram, K.S.Syed Ali Abtheen, R.Sujatha

A simple, rapid and precise RP-HPLC method was developed and validated for terbutaline sulfate, guaifenesin and bromhexine hydrochloride in a syrup formulation. A Phenomenex Luna C18 column, 250×4.6mm, 5μm in gradient mode, with mobile phase ammonium formate buffer pH 3.0 and acetonitrile were used. The flow rate was 1 ml/min and individual components were measured at 270nm. The retention time of terbutaline sulfate, guaifenesin and bromhexine hydrochloride was found to be around 10.79, 19.06 and 15.55 min respectively. Linearity for terbutaline sulfate, guaifenesin and bromhexine hydrochloride were in the range of 0.015 to 0.045 mg/ml, 0.005 to 0.015 mg/ml and 0.02 to 0.06 mg/ml respectively. Percentage recoveries were found to be within 99.62 to 100.97%w/w for terbutaline sulfate, 100.52 to 100.81% w/w for guaifenesin and 99.98 to 101.34% w/w for bromhexine hydrochloride respectively. The proposed method is precise, selective and rapid for estimation of terbutaline sulfate, guaifenesin and bromhexine hydrochloride.