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Isolation Of A Novel Sulfite Oxidase Gene From Codonopsis lanceolata And Analysis Of The Response Related To Abiotic Stresses

Pulla Rama Krishna, Myung Kyum Kim, Senthil Kalai Selvi, Yu-Jin Kim, Deok Chun Yang

A cDNA clone containing a sulfite oxidase(SO) gene, designated ClSO, was isolated froma medicinal plant Codonopsis lanceolata. ClSO is predicted to encode a precursor protein of 396 amino acid residues, and its sequence shares high degrees of homology with a number of other SOs. The expression of ClSO in different C.lanceolata organs was analyzed using reverse transcriptase (RT)-PCR. The results showed that ClSO expressed high in stems of intact plant, while expressed at low level in leaves and roots. In addition, the expression of ClSO under different abiotic stresses was analyzed at different time points. Among the all the various stresses, hydrogen peroxide, salt, sulfur and light triggered a significant induction of ClSO within 2-8h post-treatment. The positive responses of ClSO to the above abiotic stimuli suggested that ClSO may help to protect the plants against environmental stresses.