Абстрактный

Intermolecular attraction between DNA and fibers: A robust template for in-situ processing and PCR

Lay-Hong Seah

It is thought that the operation of strong attractive intermolecular forces that can exist between DNA and chains of natural fibers like cotton and wool have made microsatellites amenable to in-situ processing and PCR multiplexing. In this study, the binding properties of six types of fabrics of differentmonomeric compositionwithDNAwere investigated. Blood dried on cotton, rayon, nylon, wool, acrylic and polyester were tested. Multiplex PCRusing theAmpFlSTRIdentifiler kit on a 1mmto 2mmdiameter bloodstain disc successfully amplified the DNAin-situ fromcotton, rayon, nylon and wool. There was partial amplification fromacrylic and null amplification from polyester. The efficiency of the in-situ technique for multiplex genotyping ofmicrosatellites provides the capacity for (I)Genotyping large sample numbers such as inDNAdatabasingwork, (II)Aplatformfor employment of robotics, and (III) The versatility and robustness of solidmedia processing.