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Improved Method for Anther Culture Induction and Regeneration in Indica Rice Hybrids

Reda Helmy Sammour, Randa Nofal, Migahed Ammar and Abdesalam Draz

Anthers of three rice hybrids (SK2096, SK2096-1 and SK2060) having late uninucleate microspores were investigated for induction of haploid rice plants. Anthers were cultured on RZM medium and RZM medium supplemented with three concentrations of yeast extract, 0.25 gmL-1, 0.500 gm L-1, 0.75 gm L-1. The best callusing from cultured anther obtained for SK2096-1 hybrid in RZM medium supplemented with 0.25 gm L-1 yeast extract. It gave 58%callus induction. The other hybrids performed well on RZM media with the other two concentrations of yeast extract. However, the callus induction percentages obtained were less than that for SK2096- 1. For regeneration haploid plantlets anther derived calli were transferred to MS and MSR1 media. The regeneration was very poor on MS medium compared with MSR1 medium. SK2096-1 gave the highest regeneration percentages, extended from 13 % to 36.6 %. Percentage of haploid rice plants obtained was defined. The investigation showed the importance of yeast extracts in enhancing callus initiation of the indica genotypes.