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Enzymatic transformation of Panax notoginseng stem and leaves for preparation anti-tumor activity constituents

Bin-Hui Jiang, Yu-Qing Zhao, Jia-Jun Sun, Yan Zhao, Xiao-Min Hu, Li Liu

Ginsenoside bioactive compounds, namely, 20(S)-protopanaxadiol-20-O- â-D-glucopyranoside (Compound-K,C-K) and 20(S)-protopanaxadiol-20- O-â-D-glucopyranoside (compound Mc, G-Mc) were converted from the saponins separated from the stem and leaves wastes of P. notoginseng (PNLS) by the industrial enzyme. The enzymatic transformed products CK and G-Mc were extracted byD-101macroreticular resins, separated and purified by column chromatographies on silica gel and thin layer gel, and their structures were confirmed by 1H and 13C NMR analysis. Under the optimumenzymatic transformation conditions of pH5.4, 55ï‚°C, 20mgmL-1of PNLS and 10%(v/v) of ß-glucanase, 88.30mg g-1 C-K and 10.21 mg g-1GMc was produced after 48 h