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Enhancement Of Glucose Oxidsae Production From Aspergillus Niger MTCC-281

Jagdish Singh, Neelam Verma, S.S.Marwaha

Effect of polysaccharide sodium alginate, oligosaccharides oligoman nuronate(OM), oligoguluronate and n-hexadecane on glucose oxidase (GOx) production from Aspergillus niger were studied. There was 39% increase when sodiumalginate(500mg/l) at zero hourswas supplemented in the medium. With OM blocks(10%) obtained from hydrolysis of sodiumalginate with 0.1, 0.2, 0.3 and 1.0M HCL respectively there was 41, 42, 42, 41.05%increase as compare to the control.Almost similar results were obtained with the OG blocks. Small increase(24.5%) was observed with 5% n-hexadecane. Maximum enhancement(48.35%) was achieved when n-hexadecane was supplemented in combination with sodium alginate(500mg/l) at zero hours of fermentation. No significant influence was observed on fungal biomass production with enhancer.