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Development and validation of a new LC-MS/MS bio-analytical method for the determination of Curcumin in human plasma samples

Saurabh Arora, Sunny Chopra, Kanchan Kohli, Praveen Baliyan, Roop K.Khar

The objective of current investigation is to develop a method that can be suitably applied for analysis of curcumin in human plasma. There is no sensitive bioanalyticalmethod reported in literature for curcumin in human plasma yet. Hence it is of prime importance to develop amethod for estimating curcumin in biologicalmatrix with highest level of sensitivity for better estimate of pharmacokinetic parameters like AUC0 (Area under curve from zero to infinity) and Kel (Elimination rate constant) in patients.Afast, sensitive and specific LC–MS/MS bio-analytical method has been developed and validated.We achieved not only high sensitivity of 10.08g /ml as the Lower Limit of Quantification (LLOQ) but also showed dynamic linearity ranging from10-500g/ml (r2=0.9991) with acceptable precision, accuracy and recovery (as per limits set byUSFDA). Our novelmethod involves minimumextraction steps achieving an excellent recovery (more than 80% with Coefficient of Variation (CV) less than 10%). Sufficient stability was shown to allow for the completion of sample analysis in clinical trials. This validated rugged method led to analysis of large number of plasma samples in a very short span of time (due to lower retention time of 4-5 min).