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Determination of total phenolic content and antioxidant activity of Ganoderma lucidum collected from Dang district of Gujarat, India

H.A.Modi, Pooja Shah, M.D.Shukla, Suman K.Lahiri

The main aimof this study is to determine the total phenolic content (TPC) and the antioxidant activity of the four extracts (methanol, ethyl acetate, chloroform, methanol: ethyl acetate) of G. lucidum. Results clearly showed that the methanolic extract had the highest TPC followed by Methanol: ethyl acetate, chloroformand ethyl acetate extract. The antioxidant activity of the extractswas determinedwith the help of 1, 1-diphenyl-2-picrylhydrazyl (DPPH) free radical scavenging activity assay, 2,2Â’-azinobis (3- ethylbenzothiazoline-6-sulphonic acid) (ABTS) free radical scavenging activity assay and Ferric Reducing Antioxidant Power (FRAP) assay. For the DPPH assay, the lowest IC50 was shown by the methanol extract, thus having the highest antioxidant activity and for the ABTS assay, again the lowest IC50 was shown by the methanol extract. For the FRAP assay, the reducing ability of the ethyl acetate extract was found to be the highest. A positive correlation between the total phenolic content and the three antioxidant assays was observed for each extract. This in vitro study suggested that G. lucidum could be used as a potent natural antioxidant.