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Determination of Oxadiazon Herbicide Residues in Different Ecotox Samples

Murthy SVS,T entu Nageswara Rao, Kumar G, Krishna Rao N, Karri.Apparao and Seshamma S

A simple and inexpensive high performance liquid chromatography method was developed for the determination of oxadiazon residues in different ecotox mediums. The test mediums are blended water for fish, M4 Medium for Daphnia magna, OECD TG 201 medium for Alga and 20XAAP Medium for lemna. The oxadiazon residues estimated by a validated High Performance Liquid Chromatography with Ultra Violet detector (HPLCUV) at a wave length of 230 nm. The method showed recovery 91 + 5% recovery in ecotox mediums. The method has limit of quantification (LOQ) 0.05 mg/L. The proposed validated method can be applied successfully for the determination of oxadiazon residues in different aquatic solutions having pH range 5-9 and high ionic concentration.