Абстрактный

Determination of lacidipine by thin layer chromatographicdensitometric, spectrophotometric methods and modified Vierordt’s method in presence of its photodegradates

Mohammed F.EL-Tarras, Lories.I.Bebawy, Samah Abd Elsabour Mohammed

Four methods are presented for the determination of lacidipine in presence of its photodegradates. The first method was based on the determination of lacidipine in presence of its photodegradates by densitometric thin layer chromatography (TLC) measurement at 284 nm using toluene: acetone: methanol: ammonia 25 % (60:20:6:2 by volume) as mobile phase. The second method was based on the determination of the drug at the zero crossing point of its photodegradate, where first derivative (D1)measurement is carried out at 345 nm. The third method was based on the resolution of the drug and its degradate by first derivative ratio spectra (DD1)at 250 nm. The fourth method was the determination of lacidipine and its photodegradate bymodified VierordtÂÂ’s method at 274 nm and 376 nm. The proposed methods were successfully applied for the determination of lacidipine in bulk powder, in pharmaceutical formulation with good accuracy and precision. The methods have been validated by analyzing laboratory prepared mixtures containing lacidipine and its degradates without any separation steps. The results obtained were statistically compared with that of the reported method.