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Content determination of total saponins from Opuntia

Han Benyong, Chen Ying, Ren Ying, Chen Chaoyin

Objective: To establish for content determination of total saponins in Opuntia. Methods: Total saponins was determined by UV-VIS spectrophotometry after extracts of the sample had been coloured. Results: The methods was linear in the range of 0.05~0.30mg (r=0.9987), and the average recovery was 98.47%, RSD was 3.29% (n=5). Conclusion: The methods is sensitive, reliable, simple and reproducible for the determination of the contents of saponins in Opuntia.