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Construction and analysis of the AFLP reaction system of J. curcas L

Chen Ke, Jiang Qi, Zhu Jing, Zhang Yuan

The high-quality genomic DNA which was suitable for AFLP analysis was isolated by an improved CTAB method from J. curcas L. Some key factors affecting DNA extraction, restriction-ligase reaction, pre-amplification and amplification processes were studied and an optimized AFLP reaction system of J. curcas was established. Referring to the AFLP reaction system, clear DNA electrophoresis fragments of J. curcas were obtained and the result was well reproductive