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Comparative Study on Tert-Butyl Hydroperoxide Induced Chemiluminescence in Bovine, Equine and Canine Erythrocyte Lysate

Savignone C, Ventura B, Mattioli G and Palacios A

The present investigation was carried out to analyze changes in the time course of chemiluminescence intensity in bovine, equine and canine suspension of lysates erythrocytes induced for incubation with t-butyl hydroperoxide (t-BHP). The addition of t-BHP to canine, equine and bovine suspension of lysates erythrocyte resulted in the peroxidation as evidenced by the emission of light. Light emission shows a characteristic kinetic, when the samples are added with t-BHP the emission increased quickly to reach a maximal level after about 10 min for all species, then declined with time. The total chemiluminescence during incubation of suspension of lysates erythrocyte in the three species with t-BHP was higher in canine and equine than in bovine lysed red cells. The main conclusion of this study is that the determination of chemiluminescence in suspension of lysates erythrocyte is a sensitive assay applied to detect the existence of oxidative stress associated to experimental pathological situations.