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Cleome droserifolia (Cleomaceae) methanolic extract alleviates alloxan-induced oxidative stress and â-cell damage in rat pancreas

M.A.Nagy, S.A.Mohamed

Ethnopharmacological relevance: Cleome droserifolia is used in egyptien traditionalmedicine for treatment of diabetesmellitus. The aimof the present studywas to evaluate the possible protective effects of Cleome droserifolia methanolic extract (CDE) against pancreas â-cellsÂÂÂÂ’ damage and antioxidant defense systems in alloxan induced diabetes rats.Materials andmethods: Experimental diabeteswas induced by a single dose of alloxan (150 mg/kg) administered by intraperitoneal way. The oxidative stress was measured by tissue MDA levels, reduced glutathione (GSH) content and by enzymatic activities of superoxide dismutase (SOD), catalase (CAT), and glutathione peroxidase (GPx) in pancreas. Biochemical observations were further substantiated with histological examination of pancreas. Results: The increase in blood glucose and MDA levels with the decrease in GSH content and in enzymatic activities were the salient features observed in diabetic rats. Administration of CDE (0.31 g/kg bw/day, orally) for 30 days caused a significant reduction in blood glucose and MDA levels in alloxan treated rats when compared with diabetic rats. Furthermore, diabetic rats treated with CDE showed a significant increase in the activities of both enzymatic and non-enzymatic antioxidants when compared to those of diabetic rats. Degenerative changes of pancreatic â-cells in alloxan treated rats were minimized to near normal morphology by administration of CDE as evidenced by histopathological examination. Conclusion: Results clearly indicate that Cleome droserifolia treatment exerts a therapeutic protective nature in diabetes by decreasing oxidative stress and pancreatic â-cellsÂÂÂÂ’ damage which may be attributed to its antioxidative potential.