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Biosorption of heavy metals by using free and immobilized cells of Pseudomonas aeruginosa and Klebsiella pneumoniae

M.Muthuselvam, S.Arunkumar

Various methods which are commonly used for the wastewater treatment for heavy metals, In the present work the free and immobilized cells of Pseudomonas aeruginosa., Klebsiella pneumoniae, were studied as biosorbent materials for the removal of Zn, Mg, and Ba from aqueous solution. . The both free cell and immobilized cells of Pseudomonas aeruginosa showed maximum sorption of heavy metals (Zn, Mg, and Ba) compared with Klebsiella pneumoniae. In this study % of sorption capability was calculated by using Gravimetric method. Pseudomonas aeruginosa has high ability of heavy metal sorption such as Zn, Mg, and Ba compared with Klebsiella pneumoniae sorption ability of Zn, Mg and Ba. The reaction mixtures were incubated with varied initial concentration of biomass to verify the change on the sorption capacity of the bacterial biomass. The biosorption concentration was increased simultaneously heavy metal sorption ability also increased when the biomass concentration increased. The different parameters like biomass concentration, pH, contact time and temperature were also analyzed. Hence these organisms are used for the wastewater treatment, which is a cheapest method, due to the biosorption capacity of the organisms. The biosorption performance of Pseudomonas aeruginosa and Klebsiella pneumoniae can be implemented practically for the treatment of wastewater.