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Assessment of protein stability by measuring the thermal denaturation temperature (Tm): Key considerations

Jay Kant Yadav

Assessment of thermodynamic stability of a protein is an essential step toward its characterization. The present study elucidates the significance of incubation conditions, such as retention time, on the accurate determination of thermal denaturation temperature (Tm) of protein.Areciprocal relationship was evident between scan rate and value of calculated Tm of two á-amylases differing in their thermal stability. Based on this observation it is suggested that the scan rate should be considered as a vital parameter while performing thermal denaturation and calculating the Tm and further analysis of data.