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Assessment of genetic fidelity of micropropagated clones of strawberry genotypes using EST-SSR markers

Vandana, Nand Kishor Sharma, Mithilesh Kumar

Micropropagated plants of two commercially important genotypes of strawberry (Fragaria x ananassa Duch.) viz. Festival and Sweet Charlie were subjected to evaluation of genetic uniformity using EST-SSRmarkers. Out of fifteen primers screened, twelve primers showed amplification and the PCR profiles obtained were found to be clear, reproducible and monomorphic. The regenerated plantlets were identical based on ESTSSR analysis and true-to-type character of the tissue cultured plants was established. The results concluded that the tissue culture raised plantlets of strawberry were genetically identical and clonally uniform. Thus, a comprehensive micropropagation protocol was developed for the two genotypes of strawberry.