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Assay for Screening of Acetylcholinesterase Inhibitors

S. Manasa, V. Sharada and A. B. Raju

A new experimental model using isolated frog rectus abdominus muscle preparation, acetyl choline and brain homogenate was used to screen acetylcholinesterase inhibitors. When brain homogenate was given along with sub maximal dose of acetylcholine, the response of acetyl choline was found to be reduced and this can be attributed to the presence of acetylcholinesterase enzyme in rat brain homogenate. When revastigmine a proved acetylcholinesterase inhibitor was added to the above mixture of ach and brain homogenate the responses recorded were similar to the sub maximal dose of acetylcholine and effect of brain homogenate was effectively nullified.