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Asensitive and selective liquid chromatographicmethod for the determination of zolpidemfromhuman plasma using fluorescence detector

H.S.Karmalkar, P.V.Suvarna, M.S.Bagul, A.C.Nimkar, R.D.Shah

Asimple, sensitive, selective LCmethod with a short run time (4.5 min) was developed and validated for the determination of Zolpidem from human plasma using fluorescence detector (excitation 254 nm and emission 390 nm). The sampleswere deproteinised usingmethanol.After deproteinization the analyte and the internal standard (Etodolac) were separated on a C18 column using 5mM Potassium Dihydrogen Phosphate:Acetonitrile (65:35 v/v: pH 7.5 with triethylamine) as mobile phase. The analyte exhibited a linear range of 2.08-246.43 ngmL-1.Acceptable Precision andAccuracywere obtained for the concentrations over the standard curve range. The Intraday Accuracy ranged between 91.25-111.21 % with a precision of 2.03- 5.77%. The Inter-Day accuracy was between 95.39-111.21% with a precision of 2.13-3.58%. The validated method was successfully applied for the quantitation of Zolpidemfromhuman plasma samples in a pharmacokinetic study.