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Analysis of the genetic structure of iranian indigenous raeni cashmere goat populations using microsatellite markers

Nahid Askari, Amin Baghizadeh, Mohamadreza Mohamad Abadi

In this paper, genetic diversitywas evaluated in six Iranian indigenous Raeni Cashmere goat populations using eightmicrosatellitemarkers. Twenty individuals were randomly selected from each population. Total genomic DNA was extracted from the whole blood using optimized and modified saltingout method. The extracted DNA was amplified through polymerase chain reaction (PCR) of the eightmicrosatellite loci used in this study showed that all of the loci were polymorph within the six populations. Values of both polymorphic information content (PIC) and heterozygosity were high and the mean observed heterozigositywas 0.80±0.07. A Neighbor-joining (N-J) diagram based on Nei,s genetic distances, yielded relationships of shared alleles between single individuals. The some specific alleles were shown to be important in the construction of the population structure and 19 specific alleles were shown to be important in the construction of the population structure.